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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in several tumors, which ends up in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in promoting mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not yet readily available.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide commonly consists of the epitope identified via the antibody.
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This guideline outlines further doable optimization of mobile and immunoassay parameters to be certain the best possible effects are...
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This guidebook outlines further more attainable optimization of mobile and immunoassay parameters to be certain the absolute best benefits are...
Antibodies certain to the blocking peptide no longer bind towards the epitope around the target protein. By read more evaluating the staining within the blocked antibody vs . the antibody by itself, you can see which staining is certain.
Goal Info Cyclin E belongs for the very conserved cyclin spouse and children, whose members are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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